| The effect of phospholipid composition of reconstituted HDL on its cholesterol efflux and anti-inflammatory properties[S] | |
| Source | Journal of Lipid Research |
|---|---|
| Author | Anna Schwendeman, Denis O. Sviridov, Wenmin Yuan, Yanhong Guo, Emily E. Morin, Yue Yuan, John Stonik, Lita Freeman, Alice Ossoli, Seth Thacker, Salena Killion, Milton Pryor, Y.Eugene Chen, Scott Turner, Alan T. Remaley |
| Country | USA |
| Publication Date | 2015-09 |
| [Abstract] The goal of this study was to understand how the reconstituted HDL (rHDL) phospholipid (PL) composition affects its cholesterol efflux and anti-inflammatory properties. An ApoA-I mimetic peptide, 5A, was combined with either SM or POPC. Both lipid formulations exhibited similar in vitro cholesterol efflux by ABCA1, but 5A-SM exhibited higher ABCG1- and SR-BI-mediated efflux relative to 5A-POPC (P < 0.05). Injection of both rHDLs in rats resulted in mobilization of plasma cholesterol, although the relative potency was 3-fold higher for the same doses of 5A-SM than for 5A-POPC. Formation of preβ HDL was observed following incubation of rHDLs with both human and rat plasma in vitro, with 5A-SM inducing a higher extent of preβ formation relative to 5A-POPC. Both rHDLs exhibited anti-inflammatory properties, but 5A-SM showed higher inhibition of TNF-α, IL-6, and IL-1β release than did 5A-POPC (P < 0.05). Both 5A-SM and 5A-POPC showed reduction in total plaque area in ApoE−/− mice, but only 5A-SM showed a statistically significant reduction over placebo control and baseline (P < 0.01). The type of PL used to reconstitute peptide has significant influence on rHDL's anti-inflammatory and anti-atherosclerosis properties. Original Paper Download URL : https://www.jlr.org/article/S0022-2275(20)35502-4/fulltext |
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